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The Chromatin-Based Epigenome in Development and Aging
- Bruce H. Howard, MD, Head, Section on Human Genetics
- Valya Russanova, PhD, Staff Scientist
- Irene Stevens, BS, Postbacccalaureate Intramural Research Training Award Student
The normal human lifespan is marked by a complex series of developmental transitions, with relative stability during adulthood and, ultimately, a gradual decline in viability. Clock-like mechanisms presumably underlie the developmental events that occur through childhood and adolescence. Further, instabilities in such mechanisms are likely to be an integral part of the aging process and to contribute to many common degenerative diseases of later life. A breakthrough in the area of developmental transitions would have remarkable implications, and our research in this area reflects today's widespread interest in the rapidly evolving field of epigenomics as the key to further progress.
Whole genome surveys of epigenome structure
The past year saw continued application of next-Gen sequencing approaches to studies that use human tissues from several clinical sources. We procure human skin fibroblasts from newborns and adults, as needed, under a protocol approved by the NICHD Institutional Review Board. Through collaborative work with Alan DeCherney and James Segars, we obtain human ovarian granulosa cells harvested in association with ART (assisted reproductive technology) services at Shady Grove Hospital (Gaithersburg, MD).
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A) An example of a genomic location (n) and its multiple associated features: gene A, gene B isoform 1, gene B isoform 2. Each feature is assigned with an integer code (e.g., 185, 6, 22) in the concatenated annotation file.
B) The set of algorithms for locating n and recovering its associated features. Three files are required: annotation file (.znt4), concatenated file (.coc), and index file. The .znt4 and .coc files are opened in binary mode, and contain information in integer (4-byte units), and composite integer + character (5-byte units), respectively.
• Step 1. Move file pointer to chrN.znt4 file position (= n*4), to obtain address in chrN.coc file, eg. 2120.
• Step 2. In chrN.coc file, move pointer to file address 2120, which is associated with a 3-link chain. Each link represents a feature. Links are separated by a “continue” integer “1”; each chain is terminated by an integer “0”.
• Step 3. Using index file, the integer codes recovered from Step 2 can be related to respective features.
With both of these experimental systems, we investigate developmental and age-related changes in chromatin structure, DNA methylation, and gene expression. In mammalian cells, cis-dependent epigenetic states are, of course, maintained by both chromatin structure and DNA methylation. We assess chromatin states with respect to histone acetylation and histone methylation patterns, as well as the topologies of these patterns extending over domains that typically encompass one or more genes. In previous experiments, we characterized DNA methylation at the whole-genome level (MeDIP-seq, MethylCap-seq), as well as at nucleotide resolution over gene-size domains (for the latter we optimized the rapid design and testing of tiled, nested primer pairs suitable for bisulfite-modified DNA). Of particular interest are interactions between the chromatin- (e.g., histone H3-K27 methylation) and DNA methylation–based components of epigenetic control.
Recent experiments with granulosa cells employed RRBS (Reduced Representation Bisulfite Sequencing) and RNA-seq to characterize methylation patterns and gene expression, respectively. By combining two or more approaches, whole-genome surveys can be focused to explore epigenome features that underlie changes in gene function. Along these lines, bioinformatics analyses revealed that CpG-enriched regions localized near gene 3’ ends may play an unexpected role in controlling gene expression changes that accompany decreased responsiveness of human granulosa cells to hormonal (ART-based) stimulation. Such regions appear to have a particularly strong influence on the regulation of small transcription units such as the AMH (anti-Mullerian hormone) gene, which has been extensively studied as an important feature of declining ovarian function.
Given the rapid progress of epigenomics and the very large data sets generated by linking next-Gen sequencing–based approaches to chromatin immunoprecipitation (ChIP-seq), bisulfite analysis (RRBS), and RNA expression studies (RNA-seq), continuing refinement of bioinformatics tools is essential. We constantly improve and extend our genome annotation, pattern recognition, and pattern comparison algorithms. For topology-based mapping of chromatin patterns, we have also collaborated with the Section on Chromatin and Gene Expression (headed by David Clark) on the small yeast genome, which permits highly deepened sequencing coverage, and thus hightened resolution patterns. Over the past year, a particularly important advance was development of new bioinformatic tools to efficiently link patterns in RNA expression data sets with epigenome features (Figure 1).
Our chromatin-related results to date indicate that genes subject to both differentiation and developmental controls depend on the three-dimensional topology of the genome and are sensitive to the remodeling of higher-order chromatin structures. We hope to complete studies on large (on the order of 100 kb) domains over which histone acetylation or histone H3–K27 patterns are altered, as well as on control elements that may assume non–B DNA conformations. DNA methylation–related results indicate that a little-studied subcompartment of the genome consisting of small sets of regions with high homology—either within gene clusters or dispersed gene families—may be substantially enriched for post-natal developmental- and age-related epigenome remodeling. If our hypothesis is correct, such regions may provide clock-like functions of considerable importance.
The long-term goal for this field is to generalize paradigms of dynamic postnatal epigenome structure so as to address in new ways a range of current problems in maternal reproductive health, pediatrics, and age-related disease.
- Supplementary funding from the Program in Reproductive and Adult Endocrinology, NICHD
- Scientific Director's challenge award
- Salpea P, Russanova V, Hirai T, Sourlingas TG, Sekeri-Pataryas KE, Romero R, Epstein J, Howard BH. Postnatal development- and age-related changes in DNA methylation patterns in the human genome. Nucleic Acids Res 2012;40:6477-6494.
- Cole HA, Howard BH, Clark DJ. Genome-wide mapping of nucleosomes in yeast using paired-end sequencing. Methods Enzymol 2012;513:145-168.
- Nagarajavel V, Iben JR, Howard BH, Maraia RJ, Clark, DJ. Global 'bootprinting' reveals the elastic architecture of the yeast TFIIIB-TFIIIC transcription complex in vivo. Nucleic Acids Res 2013;41:8135-8143.
- David J. Clark, PhD, Program in Genomics of Differentiation, NICHD, Bethesda, MD
- Alan DeCherney, MD, Program in Reproductive and Adult Endocrinology, NICHD, Bethesda, MD
- Jonathan Epstein, MS, Molecular Genomics Laboratory, NICHD, Bethesda, MD
- James Segars, MD, Program in Reproductive and Adult Endocrinology, NICHD, Bethesda, MD
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